Effect of heat treatment on metallothionein isoforms using capillary zone electrophoresis
نویسندگان
چکیده
Metallothionein (MT) is an inducible protein, with a molecular mass of about 7,000. Although MT can bind with metals such as Zn, Cu, Cd, and Ag, Zn7-MT is commonly induced in the organs except the injection of Cu, Cd or Ag [1]. MT exists as two major isoforms MT-1 and MT-2 in mammalian species, and both MTs vary by a few amino acid residues and have consequently very similar hydrophobicities and electric charges. Both MTs are induced coordinately in most organs by same stimuli [1,2].
منابع مشابه
Detection and identification of rabbit liver metallothionein-2 subisoforms by capillary zone electrophoresis - inductively coupled plasma mass spectrometry and microbore HPLC - electrospray mass spectrometry
To date, most of works concerning metallothioneins have been referring either to the MT-fraction (as isolated by gel filtration) or to two MT-isoforms resulting from the further fractionation of the MT-fraction by anion-exchange chromatography into MT-1 (eluting first) and MT-2 (eluting second). However, a further characterization of these fractions by reversed-phase HPLC [1] and capillary zone...
متن کاملModern bioanalysis of proteins by electrophoretic techniques.
In 1957, protein rich in cysteine able to bind cadmium was isolated from horse kidney and named as metallothionein according to its structural properties. Further, this protein and metallothionein-like proteins have been found in tissues of other animal species, yeasts, fungi and plants. MT is as a potential cancer marker in the focus of interest, and its properties, functions, and behavior und...
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